Live imaging technology represents a paradigm shift in how we study biological processes, from the traditional static 2D snapshots (i.e. histology) to dynamic, 3D view of the living specimen as processes unfold in real time. While whole-body imaging modalities such as MRI and CT have been highly successful in the clinical setting, mechanistic studies of biological processes such as disease progression and response to therapy in preclinical (animal) models require cellular details that are beyond the resolution of these whole-body imaging modalities. We have established a unique in vivocellular-level imaging resource to facilitate and accelerate collaborative biomedical research. This resource has strong imaging expertise as well as cutting-edge instrumentation that are developed in house and not commercially available. We develop and use confocal and multiphoton fluorescence imaging as well as other modalities. In particular, we have developed miniature high-resolution endomicroscopy that allows us to access internal organs minimally invasively in vivo and over time.
Active Collaborators (a partial list):
Gilles Benichou, PhD, Immunology, MGH, HMS
Robert Colvin, Pathology, MGH, HMS
Paolo Decuzzi, PhD, Nanomedicine, Methodist Hospital, Houston.
Mauro Ferrari, PhD, Methodist Hospital, Houston.
Dai Fukumura, MD, Vascular Biology, Oncology, MGH, HMS
Rakesh Jain, PhD, Vascular Biology, Oncology, MGH, MHS
Lee Kaplan, MD, Gastroenterology, MGH, HMS
Sunghoon Kim, PhD, Pharmacology, SNU
James Kobler, PhD, Laryngology, MGH, HMS
Gou-Young Koh, MD, PhD, KAIST
Maria Koulmanda, PhD, Surgery, BIDMS
Raju Kucherlapati, PhD, Genetics, BWH, HMS
Atsushi Mizoguchi, MD, PhD, Pathology, MGH, HMS
Alexander van Oudenaarden, PhD, Physics/Biology, MIT
Roberto Pineda, MD, Ophthalmology, MEEI, HMS
Terry Strom, MD, Immunology, BIDMS, HMS
Ramnik Xavier, MD, PhD, Gastroenterology, MGH, HMS
- In vivo microscopy in mice