Wound healing and localized infection is being studied in animal models. The use of optical imaging of infections caused by genetically-engineered bioluminescent microbes allows testing of antimicrobial therapies in real time.
Our expertise in this area has allowed us to collaborate with a company that prepared a chitosan acetate bandage initially as a hemostatic dressing (HemCon). We were able to show that it as highly effective against infections in wounds and burns, could be advantageously combined with nanocrystalline silver and accelerated wound healing in both infected and uninfected wounds. A recent study looked at gene expression array analysis in mouse models of burns.
We have collaborated with several additional investigators using our expertise of in vivo bioluminescence monitoring of infections in small animal models.
A recent project is looking at similar studies using Hydrofera Blue and antimicrobial dressing containing methylene blue and gentian violet.
Shi L, Takahashi K, Dundee J, Shahroor-Karni S, Thiel S, Jensenius JC, Gad F, Hamblin MR, Sastry KN, Ezekovitz RA. Mannose-binding lectin deficit mice are susceptible to infection with staphylococcus aureus. J Exp Med 2004; 199(10):1379-90.
Møller-Kristensen M, Shi L, Ip E,. Gowda LD, Hamblin MR, Thiel S, Jensenius JC, Ezekowitz RA, Takahashi K. Deficiency of mannose-binding lectin greatly increases susceptibility to post-burn infection with Pseudomonas aeruginosa. J Immunol, 2006, 176(3):1769-1775.
Møller-Kristensen M, Hamblin MR, Thiel S, Jensenius JC, Takahashi K. Burn injury reveals altered phenotype in mannan-binding lectin deficient mice. J Invest Dermatol, 2007, 127: 1524-1531.
Apidianakis Y, Mindrinos MN, Xiao W, Tegos GP, Papisov MI, Hamblin MR, Davis RW, Tompkins RG, and Rahme LG. Involvement of skeletal muscle gene regulatory network in susceptibility to wound infection following trauma. PLoS ONE, 2007, 2:e1356-1365. PMC2131783
Burkatovskaya M, Tegos GP, Swietlik E, Demidova TN, Castano AP, Hamblin MR. Use of chitosan bandage to prevent fatal infections developing from highly contaminated wounds in mice. Biomaterials. 2006 Aug;27(22):4157-4164.
Burkatovskaya M, Castano AP, Demidova-Rice TN, Tegos GP, Hamblin MR. Effect of chitosan acetate bandage on wound healing in infected and non-infected wounds in mice. Wound Repair Regenerat, 2008, 16(3):425-431.
Dai T, Tegos GP, Burkatovskaya M, Castano AP, Hamblin MR. Chitosan acetate bandage as a topical antimicrobial dressing for infected burns. Antimicrob. Agents Chemother. 2009, 53(2):393-400.
Dai T, Huang YY, Sharma SK, Hashmi JT, Kurup DB Hamblin MR. Topical Antimicrobials for Burn Wound Infections. Recent Patents on Anti-Infective Drug Discovery, 2010, 5(2):124-51.
Huang L, Dai T, Xuan Y, Tegos GP, Hamblin MR. Synergistic combination of chitosan acetate with nanoparticle silver as a topical antimicrobial: efficacy against bacterial burn infections. Antimicrob Agents Chemother. 2011, Jul;55(7):3432-8.
Dai, T., Kharkwal GB, Tanaka M, Huang YY, Bil de Arce VJ, Hamblin MR. Animal models of external traumatic wound infections. Virulence. 2011. 2:(4), 296-315;
Dai T, Tanaka M, Huang YY, Hamblin MR. Chitosan preparations for wounds and burns: antimicrobial and wound healing effects. Expert Rev Anti-Infect Ther. 2011, 9(7):857-879.
Apidianakis Y, Que YA, Xu W, Tegos GP, Zimniak P, Hamblin MR, Tompkins RG, Xiao W, Rahma LG. Down-regulation of glutatione S-transferase alpha 4 (hGSTA4) in the muscle of thermally injured patients is indicative of susceptibility to bacterial infection. FASEB J, 2012 Feb;26(2):730-7.
Demidova-Rice TN, Wolf L, Deckenback J, Hamblin MR, Herman IM. Human Platelet-Rich Plasma- and Extracellular Matrix-Derived Peptides Promote Impaired Cutaneous Wound Healing In Vivo. PLoS ONE, 2012, 7 (2), e32146
Demidova-Rice TN, Hamblin MR, Herman IM. Acute and Impaired Wound Healing: Pathophysiology and Current Methods for Drug Delivery, Part 1: Normal and Chronic Wounds: Biology, Causes, and Approaches to Care. Adv Skin Wound Care, 2012; 25:304– 14
Baxter RM, Dai T, Kimball J, Wang E, Hamblin MR, Wiesmann WP, McCarthy SJ and Baker, SM. Chitosan dressing promotes healing in third degree burns in mice: Gene expression analysis shows biphasic effects for rapid tissue regeneration and decreased fibrotic signaling. J Biomed Mater Res A, 2012. DOI: 10.1002/jbm.a.34328
Demidova-Rice TN, Hamblin MR, Herman IM. Acute and Impaired Wound Healing: Pathophysiology and Current Methods for Drug Delivery, Part 2: Role of Growth Factors in Normal and Pathological Wound Healing: Therapeutic Potential and Methods of Delivery. Adv Skin Wound Care. 2012 Aug;25(8):349-370.
Hamrahi V, Hamblin MR, Jung W, Benjamin JB, Paul KW, Fischman AJ, Tompkins RG, Carter EA. Gram-negative bacterial infection in thigh abscess can migrate to distant burn depending on burn depth. Interdiscip Perspect Infect Dis. 2012;2012:567140