Date Prepared:

12/19/08

Name:

Bryan P. Hurley Ph.D.

Office Address:

Department of Pediatrics
Mucosal Immunology Laboratory
Massachusetts General Hospital and Harvard Medical School
114 16th Street 114-3503
Charlestown, MA. 02129

Work Phone:

(617) 726-3101

Work FAX:

(617) 726-4172

Work E-Mail:

bphurley@partners.org

Education

1994

B.A.

Biology

College of the Holy Cross

2001

Ph.D.

Immunology

Tufts University Sackler School of Graduate Biomedical Sciences (Thesis advisor, David W.K. Acheson M.D.)

A. Positions, Honors, and activities

Postdoctoral Training

2001-2002

Research Fellow

Mucosal Immunology

Children’s Hospital

Microbiology and Molecular Genetics

Harvard Medical School (Post-doctoral mentor, Beth A. McCormick Ph.D.)

2002-2004

Research Fellow

Mucosal Immunology

Massachusetts General Hospital

Microbiology and Molecular Genetics

Harvard Medical School (Post-doctoral mentor, Beth A. McCormick Ph.D.)

Faculty Academic Appointments

2004-2007

Instructor

Pediatrics

Harvard Medical School

2007-

Assistant Professor

Pediatrics

Harvard Medical School

Appointments at Hospitals/Affiliated Institutions

2004-2007

Research Associate

Pediatric Service

Massachusetts General Hospital

2007-

Assistant Microbiologist

Pediatric Service

Massachusetts General Hospital

Honors

2008

Cystic Fibrosis Foundation Research Grant Recipient

2008

Research Scholar Development Award

2003

Ruth L. Kirschstein National Research Service Award Individual Fellowship

Professional Societies

2004 -

Member

American Society for Microbiology

2004 -

Member

Society for Mucosal Immunology

Professional Activities

2008-

Laboratory Safety Officer

2006-

Invited peer reviewer for multiple granting agencies

2006-

Invited peer reviewer for various research journals

2002-

Invited Speaker to several Research Departments (approximately 1 / year)

2003-

Co-Instructor of Molecular Cell Biology: Massachusetts General Hospital

2000

Instructor of Immunology for pre-matriculating medical students: Tufts University

1999-2000

Graduate school admissions committee: Tufts University

B. Peer-reviewed Publications

Original Research

1.

Fischer, C.P., Bode, B.P., Hurley, B.P. Souba W.W. Alterations in oxidative metabolism and glutamine transport support glucose production in the tumor-influenced hepatocyte. J Surg Res. 1997; 69(2):379-84.

2.

Hurley, B.P., Jacewicz, M., Thorpe, C.M., Lincicome, L.L., King, A.J., Keusch, G.T., Acheson, D.W.K. Shiga toxins 1 and 2 translocate differently across polarized intestinal epithelial cells. Infect Immun. 1999; 67(12):6670-7.

3.

Thorpe, C.M., Hurley, B.P., Lincicome, L.L., Jacewicz, M.S., Keusch, G.T., Acheson D.W.K. Shiga toxins stimulate secretion of interleukin-8 from intestinal epithelial cells. Infect Immun. 1999; 67(11):5985-93.

4.

Thorpe, C.M., Flaumenhaft, R., Hurley, B., Jacewicz, M., Acheson, D.W.K., Keusch, G.T. Shiga toxins do not directly stimulate alpha-granule secretion or enhance aggregation of human platelets. Acta Haematol. 1999; 102(1):51-5.

5.

Hurley, B.P., Thorpe, C.M., Acheson, D.W.K. Shiga toxin translocation across intestinal epithelial cells is enhanced by neutrophil transmigration. Infect. Immun. 2001; 69(10):6148-55.

6.

Thorpe, C.M., Smith, W.E., Hurley, B.P., Acheson D.W.K. Shiga toxins induce, superinduce, and stabilize a variety of C-X-C chemokine mRNA in intestinal epithelial cells resulting in increased chemokine expression. Infect. Immun. 2001; 69(10):6140-7.

7.

Bode, B.P., Fuchs, B.C., Hurley, B.P., Conroy J.L., Suetterlin, J.E., Tanabe, K.K., Rhoads D.B., Abcouwer, S.F., Souba, W.W. Molecular and functional analysis of glutamine uptake in human hepatoma and liver-derived cells. Am J Physiol Gastrointest Liver Physiol. 2002; 283(5):G1062-73.

8.

Mrsny, R.J., Gewirtz, A.T. , Siccardi,,D., Savidge, T.C., Hurley, B.P., Madara, JL, McCormick, B.A. Identification of hepoxilin A3 in inflammatory events: A required role in neutrophil migration across the intestinal epithelia. Proc. Natl. Acad. Sci. USA 2004; 101: 7421-7426.

9.

Spacek L.A., Hurley B.P., Acheson D.W., Granok A., Currie A., Doing K., Sears C.L. Shiga Toxin-Producing Escherichia coli as a Possible Etiological Agent of Chronic Diarrhea. Clin Infect Dis. 2004; 39(5):E46-8.

10.

Hurley, B.P., Siccardi, D., Mrsny, R.J., McComick, B.A. PMN transepithelial migration induced by Pseudomonas aeruginosa requires the eicosinoid hepoxilin A3. J Immunol. 2004; 173(9):5712-20.

11.

Hurley, B.P., Williams, N.L., McCormick, B.A.. 2006. Involvement of Phospholipase A2 in Pseudomonas aeruginosa Mediated PMN Trans-epithelial Migration. Am J Physiol Lung Cell Mol Physiol. 2006; 290(4):L703-9.

12.

Köhler, H., Sakaguchi1, T., Hurley, B.P., Kase, B.A., Reinecker, H.C., McCormick, B.A. Salmonella regulates intercellular junction proteins and facilitates transepithelial neutrophil and bacterial passage. Am J Physiol Gastro. and Liver Physiol. 2007; 293(1):G178-87.

13.

Hurley, B.P., Sin, A., McCormick, B.A.. Adhesion Molecules Involved in Hepoxilin A3 Mediated PMN Trans-epithelial Migration. Clin. and Exp. Immuno. 2008; 151(2):297-305.

14.

Mumy, K.L., Bien, J.D., Pazos, M.A., Gronert, K., Hurley, B.P., McCormick, B.A. Distinct Isoforms of Phospholipase A2 mediate the ability of Salmonella enterica serotype Typhimurium and Shigella flexneri to induce the transepithelial migration of neutrophils. Infect. Immun. 2008; 76(8):3614-27.

Review Articles

1.

Thorpe CM, Hurley BP, Acheson DWK. Shiga Toxin Interactions with the Intestinal Epithelium. In, Philpott, D.J., ed. Methods in Molecular Medicine. Totowa, NJ: Humana Press, 2002: 273:263-273

2.

Hurley, BP and BA McCormick (2003) Translating tissue culture results into animal models: the case of Salmonella typhimurium. Trends in Microbiol. 11(12);562-9

3.

Hurley, BP and BA McCormick (2004) Intestinal Epithelial Defense Systems Protect Against Bacterial Threats. Current Gastroenterology Reports. 6(5):355-61

4.

Hurley, BP and BA McCormick (2008) Multiple Roles of Phospholipase A2 during Lung Infection and Inflammation. Infect. Immun. 76(6):2259-2272

Abstracts (last three years)

1.

Hurley, B.P. and B.A. McCormick. The eicosanoid prostaglandin E2 is produced by lung epithelial cells infected with Pseudomonas aeruginosa by a mechanism distinct from Hepoxilin A3 production. [Abstract] American Thoracic Society International Conference. San Diego, CA. May, 2006.

2.

Hurley, B.P. and B.A. McCormick. Role of Phospholipase A2 in Eicosanoid Production from Bacterial Infected Lung Epithelial Cells. [Abstract] FASEB Summer Research Conference. “Phospholipases”. Saxtons River, VT. July, 2006.

3.

Hurley, B.P., A.L. Goodman, P. Murphy, S. Lory, B.A. McCormick. 2007. The Two-Component Sensor Response Regulator RoxR / RoxS Plays a Role in Pseudomonas aeruginosa Adhesion to Airway Epithelial Cells. [Abstract] American Society for Microbiology. Toronto, Ontario, Canada. May, 2007.

Ph.D. Thesis

Hurley, B.P. Shiga Toxin Translocation Across Polarized Intestinal Epithelial Cells and The Role of Neutrophils in Toxin Movement [Ph.D. Thesis], Boston, MA: Tufts University Sackler School of Biomedical Science, 2001. 171 pp.

C. Research Support

Ongoing Research Support

K22 AI065425

Hurley (PI)

2/1/08 – 1/31/10

NIH/NIAID

Role: PI

“Eicosanoid Production by Infected Lung Epithelial Cells”
The main objective of this grant proposal is to identify key isoforms amongst the enzymes families; phospholipase A2 and 12-lipoxygense, which are responsible for bacterial induced neutrophil trans-epithelial migration. An additional objective is to identify bacterial factors responsible for this phenomenon.

No number

Hurley (PI)

4/1/08 – 3/31/10

Cystic Fibrosis Foundation

Role: PI

“Role of Eicosanoids andf PMN Transmigration in Cystic Fibrosis”
Expression of cystic fibrosis transmembrane receptor will be modulated to determine the effects on lung epithelial cell eicosanoid production and neutrophil trans-epithelial migration in response to Pseudomonas aeruginosa infection.

Completed Research Support

NRSA AI54054

Hurley (PI)

7/1/03 – 6/31/05

NIH/NIAID

Role: PI

“Airway Inflammation: Bacterial Epithelial Interations”
The objective of this proposal was to develop and characterize an in vitro model for airway inflammation using polarized alveolar epithelial monolayers, neutrophils and pathogenic bacteria. The key finding revealed that bacterial induced neutrophil trans-epithelial migration occurs independently of the well studied neutrophil chemo-attractant IL-8, but requires the novel eicosanoid neutrophil chemo-attractant, hepoxilin A3.

No number

McCormick (PI)

7/1/04 – 6/31/06

Cystic Fibrosis Foundation

Role: Key Personnel

“Identification of the Pseudomonas Aeruginosa factor Responsible for Inducing a Novel Inflammatory Pathway”
Using the bacterial induced neutrophil trans-epithelial migration assay, a series of Pseudomonas aeruginosa mutants was screened to gain important insight as to the nature of the bacterial factor(s) responsible for inducing neutrophil migration across lung epithelial barriers. I planned, wrote, and acquired preliminary data for this grant application under the guidance of my mentor, Dr. Beth A. McCormick Ph.D.

No number

Schloss (PI)

6/15/08 – 8/15/08

Crohn’s and Colitis Foundation of America

Role: Mentor

“The Role of Cystic Fibrosis Trans-Membrane Receptor in Intestinal Inflammation”
The purpose of this grant was to provide funding for an undergraduate summer student (Daniel E. Schloss) to perform a research project in my laboratory investigating whether epithelial cystic fibrosis trans-membrane receptor expression affects neutrophil migration across intestinal barriers.