Curriculum Vita

Bryan P. Hurley PhD
Assistant Professor of Pediatrics

B. Positions and Honors
Positions and Employment
2004-2007 Instructor of Pediatrics and Microbiology, Harvard Medical School and Massachusetts General Hospital, Boston MA
2007-present Assistant Professor of Pediatrics and Microbiology, Harvard Medical School and Massachusetts General Hospital, Boston MA
Other Experience and Professional Memberships
1999-2000 Graduate school admissions committee: Tufts University
2000 Instructor of Immunology for pre-matriculating medical students: Tufts University
2001-present Mentor to summer research fellows
2002-present Invited Guest Speaker to multiple Industry & Academic Research Units
2003-present Co-Instructor for Molecular Cell Biology course: Massachusetts General Hospital
2004-present Member, American Society for Microbiology
2004-present Member, Society for Mucosal immunology
2005-present Invited peer reviewer for multiple scientific research journals
2006-present Invited peer reviewer for multiple granting agencies devoted to funding basic research
2008-present Presenter, Pediatric GI Journal Club & Clinical Conference
2008-present Supervise post-doctoral fellows and research technicians
2008-present Director of laboratory safety for the Mucosal Immunology Laboratory
2009-present Member, Executive Committee ranking Pediatric Fellows Applicants MGHfC PEDI/GI
2010 Poster Judge, 12th International Winter Eicosanoid Conference
2011-present Member, American Physiological Society
2012 Member, American Association of Immunologists
Honors
2003 Ruth L. Kirschstein National Research Service Award Individual Fellowship (NIH/NIAID)
2008 Cystic Fibrosis Foundation Research Grant Recipient
2008 Research Scholar Development Award (NIH/NIAID)
2011 Awarded Corporate Sponsored Research Contract (Pioneer Hi-Bred International, Inc.)
2011 Awarded Formulaic Bridge Funding Support (MGH ECOR)
2012 Awarded Research Project Grant (NIH/NIAID)

C. Peer-reviewed Publications
Original Articles:

1. Fischer, C.P., Bode, B.P., Hurley, B.P. Souba W.W. Alterations in oxidative metabolism and glutamine transport support glucose production in the tumor-influenced hepatocyte. J Surg Res. 1997; 69(2):379-84. PMID: 9224411
2. Hurley, B.P., Jacewicz, M., Thorpe, C.M., Lincicome, L.L., King, A.J., Keusch, G.T., Acheson, D.W.K. Shiga toxins 1 and 2 translocate differently across polarized intestinal epithelial cells. Infect Immun. 1999; 67(12):6670-7. PMID: 10569789
3. Thorpe, C.M., Hurley, B.P., Lincicome, L.L., Jacewicz, M.S., Keusch, G.T., Acheson D.W.K.. Shiga toxins stimulate secretion of interleukin-8 from intestinal epithelial cells. Infect Immun. 1999; 67(11):5985-93. PMID: 10531258
4. Thorpe, C.M., Flaumenhaft, R., Hurley, B., Jacewicz, M., Acheson, D.W.K., Keusch, G.T. Shiga toxins do not directly stimulate alpha-granule secretion or enhance aggregation of human platelets. Acta Haematol. 1999; 102(1):51-5. PMID: 10473889
5. Hurley, B.P., Thorpe, C.M., Acheson, D.W.K. Shiga toxin translocation across intestinal epithelial cells is enhanced by neutrophil transmigration. Infect. Immun. 2001; 69(10):6148-55. PMID: 11553554
6. Thorpe, C.M., Smith, W.E., Hurley, B.P., Acheson D.W.K. Shiga toxins induce, superinduce, and stabilize a variety of C-X-C chemokine mRNA in intestinal epithelial cells resulting in increased chemokine expression. Infect. Immun. 2001; 69(10):6140-7. PMID: 11553553
7. Bode, B.P., Fuchs, B.C., Hurley, B.P., Conroy J.L., Suetterlin, J.E., Tanabe, K.K., Rhoads D.B., Abcouwer, S.F., Souba, W.W.. Molecular and functional analysis of glutamine uptake in human hepatoma and liver-derived cells. Am J Physiol Gastrointest Liver Physiol. 2002; 283(5):G1062-73. PMID: 12381519
8. Mrsny, R.J., Gewirtz, A.T. , Siccardi,,D., Savidge, T.C., Hurley, B.P., Madara, JL, McCormick, B.A.. Identification of hepoxilin A3 in inflammatory events: A required role in neutrophil migration across the intestinal epithelia. Proc. Natl. Acad. Sci. USA 2004; 101: 7421-7426. PMID: 15123795
9. Spacek L.A., Hurley B.P., Acheson D.W., Granok A., Currie A., Doing K., Sears C.L.. Shiga Toxin-Producing Escherichia coli as a Possible Etiological Agent of Chronic Diarrhea. Clin Infect Dis. 2004; 39(5):E46-8. PMID: 15356801
10. Hurley, B.P., Siccardi, D., Mrsny, R.J., McComick, B.A.. PMN transepithelial migration induced by Pseudomonas aeruginosa requires the eicosinoid hepoxilin A3. J Immunol. 2004; 173(9):5712-20. PMID: 15494523
11. Hurley, B.P., Williams, N.L., McCormick, B.A.. Involvement of Phospholipase A2 in Pseudomonas aeruginosa Mediated PMN Trans-epithelial Migration. Am J Physiol Lung Cell Mol Physiol. 2006; 290(4):L703-9. PMID: 16272174
12. Köhler, H., Sakaguchi1, T., Hurley, B.P., Kase, B.A., Reinecker, H.C., McCormick, B.A. Salmonella regulates intercellular junction proteins and facilitates transepithelial neutrophil and bacterial passage. Am J Physiol Gastro. and Liver Physiol. 2007; 293(1):G178-87. PMID: 17615177
13. Hurley, B.P., Sin, A., McCormick, B.A.. Adhesion Molecules Involved in Hepoxilin A3 Mediated PMN Trans-epithelial Migration. Clin. and Exp. Immuno. 2008; 151(2):297-305. PMID: 18005361
14. Mumy, K.L., Bien, J.D., Pazos, M.A., Gronert, K., Hurley, B.P., McCormick, B.A. Distinct Isoforms of Phospholipase A2 mediate the ability of Salmonella enterica serotype Typhimurium and Shigella flexneri to induce the transepithelial migration of neutrophils. Infect. Immun. 2008; 76(8):3614-27. PMID: 18505810
15. Hurley, B.P., Goodman, A.L., Murphy, P., Lory, S., McCormick, B.A.. The Two-Component Sensor Response Regulator RoxR / RoxS Plays a Role in Pseudomonas aeruginosa Interactions with Airway Epithelial Cells. Microbes & Infection. 2010; 12(3):190-8. PMID: 19961952
16. Hurley, B.P., Pirzai, W., Mumy, K.L., Gronert, K., McCormick B.A.. Selective Eicosanoid Generating Capacity of Cytoplasmic Phospholipase A2 in Pseudomonas aeruginosa Infected Epithelial Cells. Am J Physiol Lung Cell Mol Physiol. 2011 300(2):L286-94. PMID: 21097525

Reviews and Chapters:

1. Thorpe CM, Hurley BP, Acheson DWK. Shiga Toxin Interactions with the Intestinal Epithelium. In, Philpott, D.J., ed. Methods in Molecular Medicine. Totowa, NJ: Humana Press, 2002: 273:263-273. PMID: 12375435

2. Hurley, BP and BA McCormick (2003) Translating tissue culture results into animal models: the case of Salmonella typhimurium. Trends in Microbiol. 11(12);562-9. PMID: 14659688
3. Hurley, BP and BA McCormick (2004) Intestinal Epithelial Defense Systems Protect Against Bacterial Threats. Current Gastroenterology Reports. 6(5):355-61. PMID: 15341710
4. Hurley, BP and BA McCormick (2008) Multiple Roles of Phospholipase A2 during Lung Infection and Inflammation. Infect. Immun. 76(6):2259-2272. PMID: 18411286
Thesis:
Hurley, B.P. Shiga Toxin Translocation Across Polarized Intestinal Epithelial Cells and The Role of Neutrophils in Toxin Movement [Ph.D. Thesis], Boston, MA: Tufts University Sackler School of Biomedical Science, 2001 171 pp.
Abstracts:
(To be presented at national meetings in 2012)

1. Kubala S.A., S. Patil, M. Radano, W. G. Shreffler, and B. P. Hurley. Failure of Hepoxilins A-3 to Chemoattract Eosinophils in an in-vitro Gradient Barrier System. [Abstract]. American Academy of Allergy Asthma & Immunology Annual Meeting. Orlando, FL. March 2012.
2. Harper, M.S., W. Pirzai, B.P. Hurley, G. Ladics and B. Delaney. Assessment of an in vitro Human Intestinal Epithelial Cell Model for Evaluation of Protein Cytotoxicity. [Abstract]. Society of Toxicology Annual Meeting. San Francisco, CA. March 2012.
3. Hurley, B.P., N. Johnston, S. Nurko, and R. Rosen. The Impact of Human Pepsin on Neutrophil Migration in a Pulmonary Epithelial Model. [Abstract]. Digestive Disease Week. San Diego, CA. May 2012.
4. Tamang, D.L., W. Pirzai, G.P. Priebe, G.B. Pier, J. R. Falck, C. Morisseau, B.A. McCormick, K. Gronert, and B.P. Hurley. Hepoxilin A3 Facilitates Neutrophilic Breach of Lipoxygenase-expressing Airway Epithelial Barriers. [Abstract]. 112th General Meeting of the American Society of Microbiology. San Francisco, CA. June 2012.

D. Research Support
Ongoing Research Support
NIH/NIAID (R01 AI095338-01A1) Hurley (PI) 2/01/12 – 1/31/17
“The Role of Hepoxilin A3 in Neutrophil Breach of the Infected Airway Mucosa”
The objective of this grant is to explore the role of hepoxilin A3 using both in vitro and in vivo mouse models of acute bacterial pneumonia and to further elucidate the mechanism of hepoxilin A3 synthesis in human lung epithelial cells.
Corporate Sponsored Research Contract Hurley (PI) 05/01/11 – 04/30/12
Pioneer Hi-Bred International, Inc.
“Assessment of an in vitro Human Intestinal Epithelial Cell Model for Evaluation of Protein Cytotoxicity and Bioavailability”
The objective of this grant is to assess an in vitro model of polarized human intestinal epithelial cells for response to apical treatment with a diverse range of protein toxins.

Completed Research Support
MGH Executive Committee On Research Hurley (PI) 12/01/11 – 11/30/12
“The Role of Hepoxilin A3 in Neutrophil Breach of the Infected Airway Mucosa”
This award served as institutional bridge funding to support salary and research activities.
Cystic Fibrosis Foundation Research Grant Hurley (PI) 04/01/08 – 03/31/11
“Role of Eicosanoids and PMN Transmigration in Cystic Fibrosis”
Explored neutrophil trans-epithelial migration in response to infection utilizing CF mutant epithelial cell lines.
NIH NIAID (K22 AI065425) Hurley (PI) 02/01/08 – 05/31/10
“Eicosanoid Production by Infected Lung Epithelial Cells.”
Identified key enzymes responsible for bacterial induced neutrophil trans-epithelial migration present in human lung epithelial cell lines and gained insight into bacterial factors responsible for this phenomenon.
NIH NIAID F32 NRSA AI54054-02 (Individual) Hurley (PI) 07/01/03 – 06/30/05
“Airway Inflammation: Bacterial Epithelial Interactions”
Established and characterized an in vitro model for airway inflammation using alveolar epithelial monolayers grown on permeable Transwells, neutrophils, and pathogenic bacteria.