Date Prepared:

12/19/08

Name:

Bobby J. Cherayil

Office Address:

Department of Pediatrics
Mucosal Immunology Laboratory
Massachusetts General Hospital and Harvard Medical School
114 16th Street 114-3503
Charlestown, MA. 02129

Work Phone:

(617) 726-4170

Work FAX:

(617) 726-4172

Work E-Mail:

cherayil@helix.mgh.harvard.edu

Education

1981

MBBS

Medicine

Christian Medical College, Vellore, India

1984

MD

Pediatrics

Christian Medical College, Vellore, India

A. Positions and Honors

Professional Positions

1985-1986

Research fellow, Dept. of Mol. Biophys. & Biochem, Yale University

1986-1988

Research fellow, Whitehead Institute for Biomedical Research

1988-1992

Research fellow, Massachusetts General Hospital Cancer Center

1992-1993

Senior resident in pediatrics, Columbia-Presbyterian Medical Center

1993-1994

Assistant professor of pediatrics, Columbia University

1994-present

Associate immunologist, Massachusetts General Hospital

1994-2005

Assistant professor of pediatrics, Harvard Medical School

2005-present

Associate professor of pediatrics, Harvard Medical School

Honors and Awards

1972

OBA Gold Medal, St. Joseph’s High School, Bangalore, India

1973

National Science Talent Scholarship, New Delhi, India

1974

George Chacko Memorial Award for Best Incoming Student, Christian Medical College, Vellore, India

1977

Capt. Jaisingh Jadhav Prize in Pediatrics, Christian Medical College, Vellore, India

1980

Maria Viakulam David Gold Medal for Best Outgoing Student, Christian Medical College, Vellore, India

1980

The Raja of Panagal Gold Medal for Best Medical Graduate, University of Madras, Madras, India

1990

The Charles A. King Fellowship of the Medical Foundation, Boston, MA

1995

AGA/Sandoz Pharmaceuticals Research Scholar Award

1996

First Award, Crohn’s and Colitis Foundation of America

2006

American Society for Microbiology International Visiting Professorship

2007

Senior Research Award, Crohn’s and Colitis Foundation of America

2007

Milton Fund Award, Harvard University

B. Selected Publications

1.

Krupp, G., Cherayil, B.J., Frendeway, D., Nishikawa, S. and Soll, D. Two RNA species co-purify with RNase P from the fission yeast S. pombe. The EMBO J. 1986; 5: 1697-1703.

2.

Cherayil BJ. Personal view. Brit. Med. J. 1986; 293: 46.

3.

Cherayil, B.J., Sridharan, G., Thangavelu, C.P., Steinhoff, M., Koshi, G. and Pereira, S.M. Is a clinical diagnosis of streptococcal pharyngitis possible in the tropics? J. Trop. Ped. 1987; 33: 157.

4.

Cherayil, B.J., Krupp, G., Char, S. and Soll, D. The RNA components of S. pombe RNase P are essential for cell viability. Gene 1987; 60: 157-161.

5.

Cherayil, B.J. and Young, R. A 28 kD protein from M. leprae is a target of the human antibody response in lepromatous leprosy. J. Immunol. 1988; 141: 4370-4375.

6.

Cherayil, B.J., Weiner, S. and Pillai, S. The Mac-2 antigen is a galactose-specific lectin which binds IgE. J. Exp. Med. 1989; 170: 1959-1972.

7.

Cherayil, B.J., Chaitovitz, S., Wong, C. and Pillai, S.. Molecular cloning of a human macrophage lectin specific for galactose. Proc. Natl. Acad. Sci. USA 1990; 87: 7324-7328.

8.

Cherayil, B.J. and Pillai, S. The ω/λ 5 surrogate immunoglobulin light chain is expressed on the surface of transitional B lymphocytes in murine bone marrow. J. Exp. Med. 1991; 173: 111-116.

9.

Rosenberg, I., Cherayil, B.J., Isselbacher, K. and Pillai, S. Mac-2 binding glycoproteins: putative ligands for a cytosolic ?-galactoside lectin. J. Biol. Chem. 1991; 266: 18731-18736.

10.

Rosenberg, I., Iyer, R., Cherayil, B.J., Chiodino, C. and Pillai, S. Structure of the Mac-2 gene: splice variants encode proteins lacking functional signal peptides. J. Biol. Chem. 1993; 268: 12393-12400.

11.

Cherayil, B.J., MacDonald, K., Waneck, G. and Pillai, S. Surface transport and internalization of the membrane IgM heavy chain in the absence of the mb-1 and B29 proteins. J. Immunol. 1993; 151: 11-19.

12.

Aoki, Y., Isselbacher, K.J., Cherayil, B.J. and Pillai, S. Tyrosine phosphorylation of Blk and Fyn SH2 domain binding proteins occurs in response to antigen receptor ligation in B cells and constitutively in pre-B cells. Proc. Natl. Acad. Sci. USA 1994; 91: 4204-4208.

13.

Chaudhuri, A., Orme, S., Eilam, S. and Cherayil, B.J. CD40-mediated signals inhibit the binding of TRAF2 to the CD40 cytoplasmic domain. J. Immunol. 1997; 159: 4244-4251.

14.

Chaudhuri, A., Orme, S., Vo, T., Wang, W., and Cherayil, B.J. Phosphorylation of TRAF2 inhibits binding to the CD40 cytoplasmic domain. Biochem. Biophys. Res. Comm. 1999; 256: 620-625.

15.

Cherayil, B.J., McCormick, B.A., and Bosley, J. Salmonella -dependent regulation of inducible nitric oxide synthase expression in macrophages by the invasins SipB, SipC and SipD, and the effector SopE2. Infect. Immun. 2000; 68: 5567-5574.

16.

Cherayil, B.J. and Antos, D. Inducible nitric oxide synthase and Salmonella infection. Microbes Infect. 2001; 3: 771-776.

17.

Cherayil, B.J. and Walker, W.A. Ontogeny of the host response to enteric microbial infection. In Microbial Pathogens and the Intestinal Cell, Gail Hecht, Editor, ASM Press, Washington, D.C. 2003.

18.

Li, Q. and Cherayil, B.J. The role of Toll-like receptor 4 in macrophage activation and tolerance during Salmonella infection. Infect. Immun. 2003; 71: 4873-4882.

19.

Cherayil, B.J. How not to get bugged by bugs: mechanisms of cellular tolerance to microorganisms. Curr. Opin. Gastroenterol. 2003; 19: 572-577.

20.

Claud, E.C., Lu, L., Anton, P., Savidge, T., Walker, W.A. and Cherayil, B.J. Developmentally-regulated IκB expression in intestinal epithelium and susceptibility to flagellin-induced inflammation. Proc. Natl. Acad. Sci. USA. 2004; 101: 7404-7408.

21.

Li, Q., Cherayil, B.J. Toll-like receptor 4 mutation impairs the macrophage TNFα response to peptidoglycan. Biochem. Biophys. Res. Comm. 2004; 325: 91-96.

22.

Cherayil BJ, Sengupta N. Dispatch from India. N. Engl. J. Med. 2004; 350: 1471.

23.

Huang, F.C., Werne, A., Li, Q., Galyov, E.E., Walker, W.A., and Cherayil, B.J. Cooperative interactions between flagellin and SopE2 in the epithelial IL-8 response to Salmonella. Infect. Immun. 2004; 72: 5052-5062.

24.

Huang, F.C., Li, Q., and Cherayil, B.J. A phosphatidyl-inositol-3-kinase-dependent anti-inflammatory pathway activated by Salmonella in epithelial cells. FEMS Microbiol. Lett. 2005; 243: 265-270.

25.

Rhee S.J., Walker, W.A., and Cherayil, B.J. Developmentally-regulated intestinal expression of IFNγ and its target genes and the age-specific response to enteric Salmonella infection. J. Immunol. 2005; 175: 1127-1136.

26.

Cariappa, A., Mazo, I.B., Chase, C., Shi, H.N., Liu, H., Li, Q., Rose, H., Leung, H., Cherayil, B.J., Russell, P., von Andrian, U. and Pillai, S. Perisinusoidal B cells in the bone marrow mediate T-independent IgM responses to blood-borne microbes. Immunity. 2005; 23: 397-407.

27.

Chlosta, S., Fishman, D.S., Harrington, L., Johnson, E.E., Knutson, M.D., Wessling-Resnick, M., and Cherayil, B.J. The iron efflux protein ferroportin regulates the intracellular growth of Salmonella. Infect. Immun. 2006; 74: 3065-3067.

28.

Cherayil, B.J. NF-κB-dependent responses activated by bacterial-epithelial interactions. In: McCormick B, editor. Bacterial-Epithelial Cell Cross-Talk: Molecular Mechanisms in Pathogenesis. Cambridge University Press, New York, NY. 2005.

29.

Srikanth, C.V., Cherayil, B.J. Intestinal innate immunity and the pathogenesis of Salmonella enteritis. Immunol. Res. 2007; 37: 61-77.

30.

Weng, M., Huntley, D., Huang, I.F., Foye-Jackson, O., Wang, L., Sarkissian, A., Zhou, Q., Walker, W.A., Cherayil, B.J. and Shi, H.N. Alternatively activated macrophages in intestinal helminth infection: effects on concurrent bacterial colitis. J. Immunol. 2007; 179: 4721-4731.

31.

Harrington, L., Srikanth, C.V., Antony, R., Shi, H.N., and Cherayil, B.J. A role for natural killer cells in intestinal inflammation caused by infection with Salmonella enterica serovar Typhimurium. FEMS Immunol. Med. Microbiol. 2007; 51: 372-380.

32.

Harrington, L., Srikanth, C.V., Antony, R., Rhee, S.J., Mellor, A.L., Shi, H.N., and Cherayil, B.J. Deficiency of indoleamine 2,3-dioxygenase enhances commensal-induced antibody responses and protects against Citrobacter colitis. Infect. Immun. 2008; 76: 3045-3053.

33.

Wang, L. Johnson, E.E., Shi, H.N., Walker, W.A., Wessling-Resnick, M., and Cherayil, B.J. Attenuated inflammatory responses in hemochromatosis reveal a role for iron in the regulation of macrophage cytokine translation. J. Immunol. 2008; 181: 2723-2731.

34.

Wang, L., Cherayil, B.J. Ironing out the wrinkles in host defense: interactions between iron homeostasis and innate immunity. Submitted.

35.

Johnson, E.E., Srikanth, C.V., Sandgren, A., Harrington, L., Wang, L., Borregaard, N., Murray, M., Cherayil, B.J. Siderocalin inhibits the intracellular replication of Mycobacterium tuberculosis in macrophages. Submitted.

C. Research Support

Active support

Senior Research Award (Principal Investigator)

07/01/07 – 06/30/10

Crohn’s and Colitis Foundation of America

The role of indoleamine 2,3-dioxygenase (IDO) in intestinal inflammation
The goal of this project is to elucidate the functional role of IDO in the regulation of different types of intestinal inflammation.

Milton Fund Award (Principal Investigator)

01/01/08 – 12/31/08

William F. Milton Fund, Harvard University

Siderocalin in immune defense against tuberculosis
The goals of this project are to elucidate the mechanism by which siderocalin inhibits the growth of M. tuberculosis, and to investigate the changes in siderocalin expression during M. tuberculosis infection.

Research Grant
(Principal Investigator)

11/01/08 – 10/31/09

Broad Medical Research Program

Iron-based modulation of cytokine biosynthesis for treatment of intestinal inflammation
The goals of this project are to evaluate the ability of reagents that modulate iron homeostasis to reduce the severity of intestinal inflammation in animal models of IBD.

P01 DK33506
(Co-investigator)

09/30/05 – 09/29/10

NIH/NIDDK

Barrier function in the GI tract in health and disease
The major goal of this project is to study anti-microbial defense mechanisms in the gastrointestinal tract and how they are perturbed in disease states.

R01 DK070260
(Co-investigator)

05/01/05 – 04/30/10

NIH/NIDDK

Ontogeny of the human intestinal mucosal barrier and NEC
The goal of this project is to study developmental changes in the gastrointestinal tract and how these changes influence age-specific diseases such as necrotizing enterocolitis (NEC).

Completed Support

R01 AI48815
(Principal Investigator)

12/01/01 – 11/30/07

NIH/NIAID

Induction of macrophage iNOS by Salmonella
The goal of this project is to elucidate the mechanism by which Salmonella increases expression of the iNOS gene in macrophages.

Pilot feasibility project (Principal Investigator)

04/01/07 – 03/31/08

Harvard Clinical Nutrition Research Center

The hyperferritinemia (Hfe) gene in innate immunity
The goal of this project is to examine the impact of the changes in iron metabolism caused by Hfe deficiency on anti-microbial and inflammatory responses.

R21 AI 065619
(Principal Investigator)

07/01/05 – 06/30/08

NIH/NIAID

Mammalian metal transporters and Salmonella infection
The goal of this project is to elucidate the role of ferroportin 1 and other mammalian metal transporters in the intracellular survival of Salmonella.