Curriculum Vita
Bobby Cherayil, MD
Associate Professor of Pediatrics
  GENERAL INFORMATION
Date Prepared: July, 2008
Name: Bobby Joseph Cherayil
Office Address:
Laboratory of Mucosal Immunology,
Pediatric Gastroenterology Unit,
Massachusetts General Hospital,
Building 114, Room 3600,
16th Street,
Charlestown, MA 02129
E-mail: cherayil@helix.mgh.harvard.edu
Phone: 617-726-4170
Fax: 617-726-4172
Place of Birth: North Parur, India
Citizenship: United States of America
Education:
1981 MBBS Christian Medical College, Vellore, India - General Medicine
1984 MD Christian Medical College, Vellore, India - Pediatrics
Postdoctoral Training:
Internships, Residencies and Clinical Fellowships:
1980-1981 Rotating intern, Christian Medical College Hospital, Vellore, India
1981-1984 Resident in pediatrics, Christian Medical College Hospital,Vellore, India
1992-1993 Resident in pediatrics, Columbia-Presbyterian MedicalCenter, New York
1995- 2000 Clinical fellow in pediatric gastroenterology, Massachusetts General Hospital, Boston
Research Fellowships:
1985-1986 Research fellow, Dept. of Molecular Biophysics and Biochemistry, Yale University, New Haven
1986-1988 Research fellow, The Whitehead Institute, Cambridge
1988-1992 Research fellow, Massachusetts General Hospital, Boston
Licensure and Certification:
1991 Educational Council for Foreign Medical Graduates
1994 American Board of Pediatrics. Recertified 2001
2000 Full medical license - Board of Registration in Medicine, Massachusetts
2004 License renewal
Academic Appointments:
1985-1986 Research fellow, Dept. of Molecular Biophysics and Biochemistry, Yale University, New Haven
1986-1988 Research fellow, The Whitehead Institute, Cambridge
1988-1992 Research fellow, Massachusetts General Hospital, Boston
1992-1993 Postdoctoral residency fellow, Pediatrics Dept., College of Physicians & Surgeons, Columbia University, New York
1993-1994 Assistant Professor, Pediatrics Dept., College of Physicians & Surgeons, Columbia University, New York
1994-2005 Assistant Professor, Pediatrics Dept., Harvard Medical School, Boston
2005 - Associate Professor, Pediatrics Dept., Harvard Medical School, Boston
2005-current - Associate Professor, Pediatrics Dept. Harvard Medical School, Boston
Hospital Appointments:
1994- Associate immunologist, Massachusetts General Hospital, Boston
2002-2006 Assistant Pediatrician, Massachusetts General Hospital, Boston
Major Committee Assignments:
Medical School
2004- Member, Harvard Medical School Committee on Immunology
Massachusetts General Hospital
2001- Member, MGH Immunology Committee
2002 Member, review committee, MGH Fund for Medical Discovery
2003-2006 - Chair, departmental committee on pediatric clinical fellowship research
2006-current – Member, Pediatric Gastroenterology Fellowship selection committee
National
2004 - Member, NIH Special Salmonella Study Section
2007- current - Member, CCFA Research Training Awards Committee
International
2003-2004 - Coordinator, international organizing committee, Third Winter Symposium, Christian Medical College, Vellore, India
Professional Societies:
2003- American Association of Immunologists
2004- American Society for Microbiology
Editorial Boards:
2000-2001 Senior editor, Cell, Molecular Cell
Reviews editor, Immunity
2003-2005 Ad hoc reviewer, Journal of Immunology
2003- Ad hoc reviwer, Journal of Leukocyte Biology
2004- Ad hoc reviewer, Infection and Immunity
2005- Associate editor, Journal of Immunology
2006-current - Ad hoc reviewer, Microbes and Infection
2008-current - Ad hoc reviewer, Cellular Microbiology
Awards and Honors:
1972 OBA Gold Medal, St. Joseph's High School, Bangalore, India.
1973 National Science Talent Scholarship, New Delhi, India.
1974 George Chacko Memorial Award for Best Incoming Student, Christian Medical College, Vellore, India.
1977 Capt. Jaisingh Jadhav Prize in Pediatrics, Christian Medical College, Vellore, India.
1980 Maria Viakulam David Gold Medal for Best Outgoing Student, Christian Medical College, Vellore, India.
1980 The Raja of Panagal Gold Medal for Best Medical Graduate, University of
Madras, Madras, India.
1990 The Charles A. King Fellowship of the Medical Foundation, Boston.
1995 AGA/Sandoz Pharmaceuticals Research Scholar Award.
1996 First Award, Crohn's and Colitis Foundation of America.
2006 - American Society for Microbiology International Visiting Professorship
2007 - Senior Research Award, Crohn’s and Colitis Foundation of America
2007 - Milton Fund Award, Harvard University
RESEARCH AND TEACHING CONTRIBUTIONS
A. Narrative Report of Research:
Research in my laboratory is broadly directed at elucidating the molecular details of mammalian innate immunity, particularly in the gastrointestinal tract. We use a combination of in vitro and in vivo approaches to analyze the host factors that influence the response to infection with Salmonella typhimurium, an important agent of acute gastroenteritis in humans. In one series of experiments, we are attempting to elucidate the effects of altered iron metabolism on macrophage function in salmonellosis. These studies have revealed a novel role for intracellular iron in the regulation of inflammatory cytokine biosynthesis, and have important implications for our understanding of host-pathogen interactions in conditions such as hemochromatosis. Other studies in the lab are directed at clarifying the role of the immunoregultory enzyme indoleamine 2,3-dioxygenase (IDO) in intestinal immunity.
In addition to my laboratory research, I have been actively involved in teaching immunology to medical students. Every year for the last 6 years, I have been a tutor and lecturer in HST175, a clinically oriented, introductory course in cellular and molecular immunology offered to first year medical students, as well as some graduate students, enrolled in the Harvard-MIT Health Science and Technology program. As a tutor in this course, I lead a group of about 15 students in patient-based discussions of basic issues in immunology, a format that allows me to make effective use of both my clinical and laboratory experience.
B. Research Funding Information:
1990-92 - The Medical Foundation. Fellow. Studies on the macrophage IgE binding protein Mac-2
1991-92 - CSIBD, MGH. Principal Investigator. Studies on the macrophage IgE binding protein Mac-2
1995-96 - American Cancer Society. Principal Investigator. The role of the CD40 binding protein in B cell activation
1995-97 - American Gastroenterology Association. Principal Investigator. Signal transduction by CD40 in B lymphocytes
1996-98 - Crohn's and Colitis Foundation of America (CCFA). Principal Investigator. CD40 signal transduction and its effects on B lymphocytes and intestinal epithelial cells
1998 - CSIBD, MGH. Principal Investigator. Serum-induced morphogenesis in Candida albicans
2004-05 - Wyeth Pharmaceuticals. Principal Investigator. Effect of dairy based ingredients on Salmonella induced inflammatory responses and intracellular signaling pathways in intestinal epithelial cells
2005-10 - NIH-NIDDK (DK33506-16A1, PI: W. Allan Walker). Investigator. Barrier function of the GI tract in health and disease
2005-10 - NIH-NIDDK (DK070260, PI: W. Allan Walker). Investigator. Ontogeny of the human intestinal mucosal barrier and NEC
2001-07 - NIH-NIAID (R01 AI48815). Principal Investigator. Induction of macrophage iNOS by Salmonella
2005-08 - NIH-NIAID (R21 AI065619). Principal Investigator. Mammalian metal transporters and Salmonella infection
2007 - Harvard Clinical Nutrition Research Center. (Pilot feasibility study). Prinicpal Investigator. The hyperferritinemia (Hfe) gene in innate immunity.
2007-10 - CCFA (Senior Research Award). Principal Investigator. The role of IDO in intestinal inflammation
2008 - Milton Fund Award. Principal Investigator. Siderocalin in immune defense against tuberculosis.
C. Current Research Activities (Bench Research):
1. Project: Activation of innate immune responses by Salmonella.
Role: Principal investigator
2. Project: Mammalian iron metabolism in bacterial infections
Role: Principal investigator
3. Project: Barrier functions of the GI tract.
Role: Co-investigator
4. Project: Developmental regulation of innate immune responses to bacteria in the gut.
Role: Co-investigator
D. Report of Teaching:
1. Local contributions:
a. Harvard Medical School courses:
1997 - Patient-Doctor II, tutor, two medical students, 7 hours contact time, 3 hours preparation time.
1998 - Immunology 200, lecturer, 20 graduate students, 1.5 hours contact time, 2 hours preparation time.
1997-current - HST175, Cellular and Molecular Immunology, tutor, 15 medical and graduate students per year, 16 hours contact time, 8 hours preparation time.
2003-current - HST175, Cellular and Molecular Immunology, lecturer, 60 medical and graduate students, 2 hours contact time, 4 hours preparation time.
b. Advisory and supervisory responsibilities:
1994-current - Post-doctoral training program in Pediatric GI and Nutrition, preceptor
2001-current - HMS Fellowship in Pediatric GI and Nutrition, preceptor
2006-current - MGH Fellowship in Pediatric Gastroenterology, preceptor
c. Advisees and trainees:
Undergraduate students
1996 - Erin O'Brien, DePauw University
1998 - Eugene Chiu, DePauw University
2000 - Jacob Bosley, DePauw University
2002 - David Scott, DePauw University
2003 - Adam Werne, DePauw University
2006 - Bryce Lynn, DePauw University
Medical students
1995 - Alice Wong, State University of New York, Stonybrook
2001 - David Antos, University of Munich, Germany
Doctoral students
2006-current - Lijian Wang, Harvard School of Public Health (jointly with Dr. Allan Walker)
Post-doctoral fellows
1996-1999 - Amit Chaudhuri, PhD, currently Senior Scientist, Genentech Corp.
2001-2004 - Erika Claud, MD, currently Asst. Prof. of Pediatrics, Univ. of Chicago
2002-2004 - Fu-Chen Huang, MD, currently Pediatric Gastroenterologist, Chang Gung Memorial Hospital, Taiwan
2003-2005 - Sue Rhee, MD, currently Asst. Prof. of Pediatrics, UCSF
2004-2005 - Sabine Chlosta, MD (MD thesis advisee, Univ. of Berlin, Germany), currently Resident in Pediatrics, SUNY, Brooklyn
2005-2006 - Reuben Antony, MD, currently Fellow in Pediatric Hematology/Oncology, UCLA
2005-2007 - C.V. Srikanth, PhD, currently Research Fellow, Massachusetts General Hospital, Boston
2. Regional, national and international contributions:
Invited presentations:
1992 - Lecture: Surface transport and endocytosis of the µm heavy chain in the absence of mb-1 and B29. FASEB Meeting, Anaheim, CA.
1992 - Lecture: Assembly and transport of membrane immunoglobulin. Indian Institute of Science, Bangalore, India.
1995 - Lecture: CD40 stimulation inhibits apoptosis and promotes cell cycle progression in the human B cell line BJAB. 8th International Congress of Mucosal Immunology, San Diego, CA.
1995 - Lecture: The role of TRAF proteins in CD40 signaling. Tata Institute of Fundamental Research, Bangalore, India.
1997 - Lecture: TRAF2 and signaling by members of the TNF receptor family. 7th Annual Symposium of the Center for the Study of Inflammatory Bowel Disease, Massachusetts General Hospital, Boston, MA.
1998 - Lecture: TRAF2 and signaling through CD40. Dept. of Biochemistry, Indian Institute of Science, Bangalore, India.
2002 - Lecture: Innate immune responses to intestinal bacteria. National Centre for Biological Sciences, Bangalore, India.
2003 - Lecture: Innate immune responses to intestinal bacteria. Renal Unit, MGH, Boston, MA.
2003 - Lecture: Toll-like receptors and the innate immune response to Salmonella typhimurium. MGH Immunology Committee, Boston, MA.
2003 - Lecture: Toll-like receptors and the innate immune response to Salmonella typhimurium. Beth-Israel Deaconess Hospital, Boston, MA.
2003 - Lecture: Toll-like receptors and the innate immune response to Salmonella typhimurium. Harvard Medical School fellowship in pediatric gastroenterology and nutrition, Childrens Hospital, Boston, MA.
2004 - Lecture: Innate immune responses to Salmonella typhimurium. Dept. of Geographic Medicine, New England Medical Center, Boston, MA.
2004 - Grand rounds: Host responses to Salmonella and what they tell us about innate immunity. Dept. of Pediatrics, MGH, Boston, MA.
2004 - Lecture: Interactions between TLR5- and SopE2-activated signals during Salmonella infection of epithelial cells. American Association of Immunologists, Washington, DC.
2004 - Lecture: Close encounters and dangerous liaisons: interactions between Salmonella and the mammalian host. Third Annual Winter Symposium, Christian Medical College, Vellore, India.
2005 - Lecture: Age-dependent changes in intestinal gene expression modulate the immune response to enteric Salmonella infection. Fifteenth Annual Symposium of the Center for the Study of Inflammatory Bowel Diseases, MGH, Boston, MA.
2006 - Lecture: An important role for IFNg in acute Salmonella-induced enteritis. Annual Meeting of the American Association of Immunologists, Boston, MA.
2007 - Lecture series and workshop: Molecular and cellular basis of anti-microbial immune responses in the gastrointestinal tract. Christian Medical College Hospital, Vellore, India.
2008 - Lecture: The physician as scientist and healer. BioMatrix monthly meeting. MIT, Boston, MA.
PART III: BIBLIOGRAPHY
Original reports:
1. - Krupp G, Cherayil BJ, Frendeway D, Nishikawa S, Soll D. Two RNA species co-purify with RNase P from the fission yeast S. pombe. EMBO J. 1986; 5: 1697-1703.
2. - Cherayil BJ, Sridharan G, Thangavelu CP, Steinhoff M, Koshi G, Pereira SM. Is a clinical diagnosis of streptococcal pharyngitis possible in the tropics? J. Trop. Ped. 1987; 33: 157.
3. - Cherayil BJ, Krupp G, Char S, Soll D. The RNA components of S. pombe RNase P are essential for cell viability. Gene 1987; 60: 157-161.
4. - Cherayil BJ, Young RA. A 28 kD protein from M. leprae is a target of the human antibody response in lepromatous leprosy. J. Immunol. 1988; 141: 4370-4375.
5. - Cherayil BJ, Weiner S, Pillai S. The Mac-2 antigen is a galactose-specific lectin which binds IgE. J. Exp. Med. 1989; 170: 1959-1972.
6. - Cherayil BJ, Chaitovitz S, Wong C, Pillai S. Molecular cloning of a human macrophage lectin specific for galactose. Proc. Natl. Acad. Sci. USA 1990; 87: 7324-7328.
7. - Cherayil BJ, Pillai S. The w/l5 surrogate immunoglobulin light chain is expressed on the surface of transitional B lymphocytes in murine bone marrow. J. Exp. Med. 1991; 173: 111-116.
8. - Rosenberg I, Cherayil BJ, Isselbacher K, Pillai S. Mac-2 binding glycoproteins: putative ligands for a cytosolic b-galactoside lectin. J. Biol. Chem. 1991; 266: 18731-18736.
9. - Rosenberg I, Iyer R, Cherayil BJ, Chiodino C, Pillai S. Structure of the Mac-2 gene: splice variants encode proteins lacking functional signal peptides. J. Biol. Chem. 1993; 268: 12393-12400.
10. - Cherayil BJ, MacDonald K, Waneck G, Pillai S. Surface transport and internalization of the membrane IgM heavy chain in the absence of the mb-1 and B29 proteins. J. Immunol. 1993; 151: 11-19.
11. - Aoki Y, Isselbacher KJ, Cherayil BJ, Pillai S. Tyrosine phosphorylation of Blk and Fyn SH2 domain binding proteins occurs in response to antigen receptor ligation in B cells and constitutively in pre-B cells. Proc. Natl. Acad. Sci. USA 1994; 91: 4204-4208.
12. - Chaudhuri A, Orme S, Eilam S, Cherayil BJ. CD40-mediated signals inhibit the binding of TRAF2 to the CD40 cytoplasmic domain. J. Immunol. 1997; 159: 4244-4251.
13. - Chaudhuri A, Orme S, Vo T, Wang W, Cherayil BJ. Phosphorylation of TRAF2 inhibits binding to the CD40 cytoplasmic domain. Biochem. Biophys. Res. Comm. 1999; 256: 620-625.
14. - Cherayil BJ, McCormick BA, Bosley J. Salmonella enterica serovar Typhimurium-dependent regulation of inducible nitric oxide synthase expression in macrophages by invasins SipB, SipC and SipD and the effector SopE2. Infect. Immun. 2000; 68: 5567-5574.
15. - Li Q, Cherayil BJ. Role of Toll-like receptor 4 in macrophage activation and tolerance during Salmonella enterica serovar Typhimurium infection. Infect. Immun. 2003; 71: 4873-4882.
16. - Claud EC, Lu L, Anton P, Savidge T, Walker WA, Cherayil BJ. Developmentally-regulated IkB expression in intestinal epithelium and susceptibility to flagellin-induced inflammation. Proc. Natl. Acad. Sci. USA 2004; 101: 7404-7408.
17. - Li Q, Cherayil BJ. Toll-like receptor 4 mutation impairs the macrophage TNFa response to peptidoglycan. Biochem. Biophys. Res. Comm. 2004; 325: 91-96.
18. - Huang FC, Werne A, Li Q, Galyov EE, Walker WA, Cherayil BJ. Cooperative interactions between flagellin and SopE2 in the epithelial IL-8 response to Salmonella. Infect. Immun. 2004; 72: 5052-5062.
19. - Huang FC, Li Q, Cherayil BJ. A phosphatidyl-inositol-3-kinase-dependent anti-inflammatory pathway activated by Salmonella in epithelial cells. FEMS Microbiol. Lett. 2005; 243: 265-270.
20. - Rhee SJ, Walker WA, Cherayil BJ. Developmentally-regulated intestinal expression of IFNg and its target genes and the age-specific response to enteric Salmonella infection. J. Immunol. 2005; 175: 1127-1136.
21. - Cariappa A, Mazo IB, Chase C, Shi HN, Liu H, Li Q, Rose H, Leung H, Cherayil BJ, Russell P, von Andrian U, Pillai S. Perisinusoidal B cells in the bone marrow mediate T-independent IgM responses to blood-borne microbes. Immunity 2005; 23: 397-407.
22. - Chlosta S, Fishman DS, Harrington L, Johnson EE, Knutson MD, Wessling-Resnick M, Cherayil BJ. The iron efflux protein ferroportin regulates the intracellular growth of Salmonella enterica. Infect. Immun. 2006; 74: 3065-3067.
23. - Harrington L, Srikanth CV, Antony R, Shi HN, Cherayil BJ. A role for natural killer cells in intestinal inflammation caused by infection with Salmonella enterica serovar Typhimurium. FEMS Immunol. Med. Microbiol. 2007; 51: 372-380.
24. - Weng M, Huntley D, Huang IF, Foye-Jackson O, Wang L, Sarkissian A, Zhou Q, Walker WA, Cherayil BJ, Shi HN. Alternatively activated macrophages in intestinal helminth infection: effects on concurrent bacterial colitis. J. Immunol. 2007; 179: 4721-4731.
25. - Harrington L, Srikanth CV, Antony R, Rhee SJ, Mellor AL, Shi HN, Cherayil BJ Deficiency of indoleamine 2,3-dioxygenase enhances commensal-induced antibody responses and protects against Citrobacter colitis. Infect. Immun. 2008; 76: 3045-3053.
26. - Wang L, Johnson EE, Shi HN, Walker WA, Wessling-Resnick M, Cherayil BJ Attenuated inflammatory responses in hemochromatosis reveal a role for iron in the regulation of macrophage cytokine translation. J. Immunol. 2008. In press.
Invited reviews and chapters:
1. - Cherayil BJ, Antos D. Inducible nitric oxide synthase and Salmonella infection. Microbes Infect. 2001; 3: 771-776.
2. - Cherayil BJ, Walker WA. Ontogeny of the host response to enteric microbial infection. In: Hecht G, editor. Microbial Pathogens and the Intestinal Epithelial Cell. Washington, DC: ASM Press; 2003. p. 333-49.
3. - Cherayil BJ. How not to get bugged by bugs: mechanisms of cellular tolerance to microorganisms. Curr. Opin. Gastroenterol. 2003; 19: 572-577.
4. - Cherayil BJ. NF-kB-dependent responses activated by bacterial-epithelial interactions. In: McCormick B, editor. Bacterial-Epithelial Cell Cross-Talk: Molecular Mechanisms in Pathogenesis. Cambridge University Press, New York, NY. 2006.
5. - Srikanth CV, Cherayil B.J. Intestinal innate immunity and the pathogenesis of Salmonella enteritis. Immunol. Res. 2007; 37: 61-77.
Clinical communications:
1. - Cherayil BJ. Personal view. Brit. Med. J. 1986; 293: 46.
2. - Cherayil BJ, Sengupta N. Dispatch from India. N. Engl. J. Med. 2004; 350: 1471.
Abstracts:
1. - Cherayil BJ, Eilam S, Orme, S. TRAF2 and TRAF3 bind to overlapping but distinguishable sites on the CD40 cytoplasmic domain. AAAAI/AAI/CIS Joint Meeting, San Francisco, CA, February 21-26, 1997.
2. - Chaudhuri A, Orme S, Cherayil BJ. Binding of TRAF2 to the cytoplasmic domain of CD40 is regulated by phosphorylation. Meeting on Tyrosine Phosphorylation and Cell Signaling. The Salk Institute for Biological Sciences, San Diego, CA, August 15-19, 1998.
3. - Claud, EC, Cherayil BJ, Walker WA. Developmental regulation of the enterocyte inflammatory response to bacteria. Annual meeting of the Society for Pediatric Research, Seattle, WA, May 3-6, 2003.
4. - Cherayil BJ, Huang FC, Werne A, Li Q, Galyov EE, Walker WA. Interactions between TLR5- and SopE2-activated signals during Salmonella infection of epithelial cells. Annual meeting of the American Association of Immunologists, Washington, DC, April 17-21, 2004.
5. - Claud EC, Anton P, Lu L, Savidge T, Cherayil BJ, Walker WA. Developmental regulation of IkB expression in the intestinal epithelium. Annual meeting of the Pediatric Academic Society, San Francisco, CA, May 1-4, 2004.
6. - Rhee SJ, Walker WA, Cherayil BJ. Developmental changes in expression of interferon g-regulated genes in the intestine contribute to age-specific differences in the response to enteric Salmonella infection. Digestive Disease Week, Chicago, IL, May 14-19, 2005.
7. - Chlosta S, Fishman DS, Harrington L, Wessling-Resnick M, Cherayil BJ. The iron efflux protein ferroportin 1 regulates intracellular growth of Salmonella. Keystone Symposium on Innate Immunity, Banff, Alberta, Feb. 15-19, 2006.
8. - Cherayil BJ, Harrington L, Antony R, Shi HN. An important role for IFNg in acute Salmonella-induced enteritis. Annual Meeting of the American Association of Immunologists, Boston, MA, May 12-16, 2006.
9. - Weng M, Huntley D, Wang L, Foye-Jackson O, Walker WA, Cherayil BJ, Shi HN. The role of helminth-induced alternatively activated macrophages in concurrent bacterial enteritis. Digestive Disease Week, Washington, DC, May 14-19, 2007.
10. - Wang L, Johnson EE, Shi HN, Walker WA, Wessling-Resnick M, Cherayil BJ. A role for iron in translational control of inflammatory cytokine expression in macrophages. Cold Spring Harbor Meeting on Gene Expression and Signaling in the Immune System. Cold Spring Harbor, NY, April 22-26, 2008.
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