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People of the MIBRC

Principal Investigators:

Bobby Cherayil, MD

Alessio Fasano, MD

Verena Göbel, MD

Bryan Hurley, PhD

Hai Ning Shi, DVM, PhD

W. Allan Walker, MD

Collaborators:

Beth McCormick, PhD

Hans-Christian Reinecker, MD

Curriculum Vitae

Bryan P. Hurley PhD

Assistant Professor of Pediatrics, Harvard Medical School

Office Address:

Department of Pediatrics
Mucosal Immunology Laboratory
Massachusetts General Hospital and Harvard Medical School
114 16th Street 114-3503
Charlestown, MA. 02129

Phone: (617) 726-3101
FAX: (617) 726-4172

Education

Positions and Honors

Positions and Employment

Other Experience and Professional Memberships

Honors

Peer-reviewed Publications

Original Articles:

  1. Fischer, C.P., Bode, B.P., Hurley, B.P. Souba W.W. Alterations in oxidative metabolism and glutamine transport support glucose production in the tumor-influenced hepatocyte. J Surg Res. 1997; 69(2):379-84. PMID: 9224411
  2. Hurley, B.P., Jacewicz, M., Thorpe, C.M., Lincicome, L.L., King, A.J., Keusch, G.T., Acheson, D.W.K. Shiga toxins 1 and 2 translocate differently across polarized intestinal epithelial cells. Infect Immun. 1999; 67(12):6670-7. PMID: 10569789
  3. Thorpe, C.M., Hurley, B.P., Lincicome, L.L., Jacewicz, M.S., Keusch, G.T., Acheson D.W.K.. Shiga toxins stimulate secretion of interleukin-8 from intestinal epithelial cells. Infect Immun. 1999; 67(11):5985-93. PMID: 10531258
  4. Thorpe, C.M., Flaumenhaft, R., Hurley, B., Jacewicz, M., Acheson, D.W.K., Keusch, G.T. Shiga toxins do not directly stimulate alpha-granule secretion or enhance aggregation of human platelets. Acta Haematol. 1999; 102(1):51-5. PMID: 10473889
  5. Hurley, B.P., Thorpe, C.M., Acheson, D.W.K. Shiga toxin translocation across intestinal epithelial cells is enhanced by neutrophil transmigration. Infect. Immun. 2001; 69(10):6148-55. PMID: 11553554
  6. Thorpe, C.M., Smith, W.E., Hurley, B.P., Acheson D.W.K. Shiga toxins induce, superinduce, and stabilize a variety of C-X-C chemokine mRNA in intestinal epithelial cells resulting in increased chemokine expression. Infect. Immun. 2001; 69(10):6140-7. PMID: 11553553
  7. Bode, B.P., Fuchs, B.C., Hurley, B.P., Conroy J.L., Suetterlin, J.E., Tanabe, K.K., Rhoads D.B., Abcouwer, S.F., Souba, W.W.. Molecular and functional analysis of glutamine uptake in human hepatoma and liver-derived cells. Am J Physiol Gastrointest Liver Physiol. 2002; 283(5):G1062-73. PMID: 12381519
  8. Mrsny, R.J., Gewirtz, A.T. , Siccardi,,D., Savidge, T.C., Hurley, B.P., Madara, JL, McCormick, B.A.. Identification of hepoxilin A3 in inflammatory events: A required role in neutrophil migration across the intestinal epithelia. Proc. Natl. Acad. Sci. USA 2004; 101: 7421-7426. PMID: 15123795
  9. Spacek L.A., Hurley B.P., Acheson D.W., Granok A., Currie A., Doing K., Sears C.L.. Shiga Toxin-Producing Escherichia coli as a Possible Etiological Agent of Chronic Diarrhea. Clin Infect Dis. 2004; 39(5):E46-8. PMID: 15356801
  10. Hurley, B.P., Siccardi, D., Mrsny, R.J., McComick, B.A.. PMN transepithelial migration induced by Pseudomonas aeruginosa requires the eicosinoid hepoxilin A3. J Immunol. 2004; 173(9):5712-20. PMID: 15494523
  11. Hurley, B.P., Williams, N.L., McCormick, B.A.. Involvement of Phospholipase A2 in Pseudomonas aeruginosa Mediated PMN Trans-epithelial Migration. Am J Physiol Lung Cell Mol Physiol. 2006; 290(4):L703-9. PMID: 16272174
  12. Köhler, H., Sakaguchi1, T., Hurley, B.P., Kase, B.A., Reinecker, H.C., McCormick, B.A. Salmonella regulates intercellular junction proteins and facilitates transepithelial neutrophil and bacterial passage. Am J Physiol Gastro. and Liver Physiol. 2007; 293(1):G178-87. PMID: 17615177
  13. Hurley, B.P., Sin, A., McCormick, B.A.. Adhesion Molecules Involved in Hepoxilin A3 Mediated PMN Trans-epithelial Migration. Clin. and Exp. Immuno. 2008; 151(2):297-305. PMID: 18005361
  14. Mumy, K.L., Bien, J.D., Pazos, M.A., Gronert, K., Hurley, B.P., McCormick, B.A. Distinct Isoforms of Phospholipase A2 mediate the ability of Salmonella enterica serotype Typhimurium and Shigella flexneri to induce the transepithelial migration of neutrophils. Infect. Immun. 2008; 76(8):3614-27. PMID: 18505810
  15. Hurley, B.P., Goodman, A.L., Murphy, P., Lory, S., McCormick, B.A.. The Two-Component Sensor Response Regulator RoxR / RoxS Plays a Role in Pseudomonas aeruginosa Interactions with Airway Epithelial Cells. Microbes & Infection. 2010; 12(3):190-8. PMID: 19961952
  16. Hurley, B.P., Pirzai, W., Mumy, K.L., Gronert, K., McCormick B.A.. Selective Eicosanoid Generating Capacity of Cytoplasmic Phospholipase A2 in Pseudomonas aeruginosa Infected Epithelial Cells. Am J Physiol Lung Cell Mol Physiol. 2011 300(2):L286-94. PMID: 21097525 
  17. Tamang D.L., Pirzai W., Priebe G..P, Traficante D.C., Pier G.B., Falck J.R., Morisseau C., Hammock B.D., McCormick B.A., Gronert K., Hurley B.P..  Hepoxilin A3 Facilitates Neutrophilic Breach of Lipoxygenase-Expressing Airway Epithelial Barriers.  J Immunol. 2012; 189(10):4960-9.  PMID:23045615
  18. Trebicka E., Jacob S., Pirzai W., Hurley B.P., Cherayil B.J..  Role of anti-lipopolysaccharide antibodies in serum bactericidal activity against Salmonella enterica serovar Typhimurium in healthy adults and children in the United States.  Clin Vaccine Immunol. 2013; In press.
  19. Kusek, M.E., Pazos, M.A., Pirzai W., Hurley, B.P..  In Vitro Co-Culture Assay to Assess Pathogen Induced Neutrophil Trans-Epithelial Migration. J. Vis. Exp. 2013; In press.

Reviews and Chapters:

  1. Thorpe CM, Hurley BP, Acheson DWK. Shiga Toxin Interactions with the Intestinal Epithelium. In, Philpott, D.J., ed. Methods in Molecular Medicine. Totowa, NJ: Humana Press, 2002: 273:263-273. PMID: 12375435
  2. Hurley, BP and BA McCormick (2003) Translating tissue culture results into animal models: the case of Salmonella typhimurium. Trends in Microbiol. 11(12);562-9. PMID: 14659688
  3. Hurley, BP and BA McCormick (2004) Intestinal Epithelial Defense Systems Protect Against Bacterial Threats. Current Gastroenterology Reports. 6(5):355-61. PMID: 15341710
  4. Hurley, BP and BA McCormick (2008) Multiple Roles of Phospholipase A2 during Lung Infection and Inflammation. Infect. Immun. 76(6):2259-2272. PMID: 18411286

Thesis:

Hurley, B.P. Shiga Toxin Translocation Across Polarized Intestinal Epithelial Cells and The Role of Neutrophils in Toxin Movement [Ph.D. Thesis], Boston, MA: Tufts University Sackler School of Biomedical Science, 2001 171 pp.

Abstracts:
(Presented at national meetings in 2012)

  1. Kubala S.A., S. Patil, M. Radano, W. G. Shreffler, and B. P. Hurley. Failure of Hepoxilins A-3 to Chemoattract Eosinophils in an in-vitro Gradient Barrier System. [Abstract]. American Academy of Allergy Asthma & Immunology Annual Meeting. Orlando, FL. March 2012.
  2. Harper, M.S., W. Pirzai, B.P. Hurley, G. Ladics and B. Delaney. Assessment of an in vitro Human Intestinal Epithelial Cell Model for Evaluation of Protein Cytotoxicity. [Abstract]. Society of Toxicology Annual Meeting. San Francisco, CA. March 2012.
  3. Hurley, B.P., N. Johnston, S. Nurko, and R. Rosen. The Impact of Human Pepsin on Neutrophil Migration in a Pulmonary Epithelial Model. [Abstract]. Digestive Disease Week. San Diego, CA. May 2012.
  4. Tamang, D.L., W. Pirzai, G.P. Priebe, G.B. Pier, J. R. Falck, C. Morisseau, B.A. McCormick, K. Gronert, and B.P. Hurley. Hepoxilin A3 Facilitates Neutrophilic Breach of Lipoxygenase-expressing Airway Epithelial Barriers. [Abstract]. 112th General Meeting of the American Society of Microbiology. San Francisco, CA. June 2012. 
  5. Liu, L., W.G. Shreffler, G. Dierksen M.E. Kusek, E. Wilsterman, K.K. Chu, B.P. Hurley, G.J. Tearney.  Visualizing neutrophil trans-epithelial migration using μOCT.  [Abstract 8565-111].  SPIE Photonics West.  San Francisco, CA.  February 2013.
  6. Pazos, M.A. and B.P. Hurley.  Hepoxilin A3 is a key driver of neutrophil migration in a model of acute P. aeruginosa infection.  Experimental Biology.  Boston, MA. April 2013
  7. Kusek, M.E, K. K. Chu, L. Liu, E. Wilsterman, G. Dierksen, W. G. Shreffler, G.J. Tearney, and B.P. Hurley.  Imaging mucosal surfaces with micro-optical coherence tomography.  [Abstract].  NASPGHAN. Chicago, IL. October 2013.

Research Support

Ongoing Research Support

NIH/NIAID (R01 AI095338-01A1)        Hurley (PI)        02/01/12 – 01/31/17
“The Role of Hepoxilin A3 in Neutrophil Breach of the Infected Airway Mucosa”
The objective of this grant is to explore the role of hepoxilin A3 using both in vitro and in vivo mouse models of acute bacterial pneumonia and to further elucidate the mechanism of hepoxilin A3 synthesis in human lung epithelial cells.

Corporate Sponsored Research Contract        Hurley (PI)        07/01/13 – 12/31/14
Pioneer Hi-Bred International, Inc.
“Assessment of an in vitro Human Intestinal Epithelial Cell Model for Evaluation of Protein Cytotoxicity and Bioavailability”      Phase III
The objective of this grant is to expand Phase I and Phase II studies using in vitro models of polarized human intestinal epithelial cells for response to apical treatment with a diverse range of protein toxins and innocuous proteins.

Cystic Fibrosis Foundation        Pazos (PI)        04/01/013 – 03/31/15
Postdoctoral Research Fellowship Award
“The role of hepoxilin A3 in neutrophil migration across lung epithelia”      
I serve as mentor for this postdoctoral fellowship awarded to Michael A. Pazos Ph.D. to explore neutrophil trans-epithelial migration using in vivo and in vitro mouse models in the context of cystic fibrosis.

Completed Research Support     

Corporate Sponsored Research Contract        Hurley (PI)         07/01/12 – 03/31/13
Pioneer Hi-Bred International, Inc.
“Assessment of an in vitro Human Intestinal Epithelial Cell Model for Evaluation of Protein Cytotoxicity and Bioavailability”      Phase II
Expanded Phase I studies using multiple in vitro models of polarized human intestinal epithelial cells for response to apical treatment with a diverse range of protein toxins.

MGH Executive Committee On Research         Hurley (PI)        12/01/11 – 11/30/12
 “The Role of Hepoxilin A3 in Neutrophil Breach of the Infected Airway Mucosa”
This award served as institutional bridge funding to support salary and research activities.

Corporate Sponsored Research Contract        Hurley (PI)         05/01/11 – 04/30/12
Pioneer Hi-Bred International, Inc.
“Assessment of an in vitro Human Intestinal Epithelial Cell Model for Evaluation of Protein Cytotoxicity and Bioavailability”
Assessed an in vitro model of polarized human intestinal epithelial cells for response to apical treatment with a diverse range of protein toxins.

Cystic Fibrosis Foundation        Hurley (PI)         04/01/08 – 03/31/11
Research Grant
“Role of Eicosanoids and PMN Transmigration in Cystic Fibrosis”         
Explored neutrophil trans-epithelial migration in response to infection utilizing CF epithelial cell lines. 

NIH NIAID (K22 AI065425)         Hurley (PI)         02/01/08 – 05/31/10
“Eicosanoid Production by Infected Lung Epithelial Cells.”                               
Identified key enzymes responsible for bacterial induced neutrophil trans-epithelial migration present in human lung epithelial cell lines and gained insight into bacterial factors responsible for this phenomenon.

Cystic Fibrosis Foundation        McCormick (PI)        04/01/04 – 03/31/06
Pilot and Feasibility Award
“Identification of the Pseudomonas aeruginosa Factor Responsible for Inducing a Novel Inflammatory Pathway”      
Screened a mutant library and identified a novel gene with potential involvement in inflammatory processes.  I served as key personnel, prepared the grant application, and designed and conducted all the experiments

NIH NIAID F32 NRSA AI54054-02 (Individual)         Hurley (PI)        07/01/03 – 06/30/05
“Airway Inflammation: Bacterial Epithelial Interactions”                       
Established and characterized an in vitro model for airway inflammation using alveolar epithelial monolayers grown on permeable Transwells, neutrophils, and pathogenic bacteria.