My laboratory has an NIH-funded program of International Collaborations in Infectious Disease Research, in collaboration with the International Centre for Diarrhoeal Disease Research in Dhaka, Bangladesh, to analyze human mucosal immune responses following natural V. cholerae infection, to analyze gene expression in V. cholerae directly in human samples, to use human immune responses following cholera to identify bacterial genes uniquely expressed during human infection, and to use this information to develop an improved cholera vaccine.
Selected recent references:
- Karlsson EK, Harris JB, Tabrizi S, Rahman A, Shylakhter I, Patterson N, O’Dushlaine C, Schaffner SF, Gupta S, Chowdhury F, Sheikh A, Shin OS, Ellis C, Becker C, Stuart LM, Calderwood SB, Ryan ET, Qadri F*, Sabeti PC*, LaRocque RC*. Natural selection in a Bangladeshi population from the cholera-endemic Ganges River delta. Sci Transl Med 2013; 5: 192ra86.
- Seed KD, Lazinski DW, Calderwood SB, Camili A. A bacteriophage encodes its own CRISPR/Cas adaptive response to evade host innate immunity. Nature 2013; 494: 489-491
- Leung DT*, Uddin T*, Xu P*, Aktar A, Johnson RA, Rahman MA, Alam MM, Bufano MK, Eckhoff G, Wu-Freeman Y, Yu Y, Sultana T, Khanam F, Saha A, Chowdhury F, Khan AI, Charles RC, LaRocque RC, Harris JB, Calderwood SB, Kovac P**, Qadri F**, Ryan ET**. Immune responses to the O-specific polysaccharide antigen in children who received a killed oral cholera vaccine compared to responses following natural cholera infection in Bangladesh. Clin Vaccine Immunol 2013; 20: 780-788.
- Charles RC, Hilaire IJ, Mayo-Smith LM, Teng JE, Jerome JG, Franke MF, Saha A, Yu Y, Kovac P, Calderwood SB, Ryan ET, LaRocque RC, Almazor CP, Qadri F, Ivers LC*, Harris JB*. Immunogenicity of a killed bivalent (O1 and O139) whole cell oral cholera vaccine, Shanchol, in Haiti. PLoS Negl Trop Dis 2014; 8:e2828.
- Leung DT, Bhuiyan TR, Nishat NS, Hoq MR, Aktar A, Rahman MA, Uddin T, Khan AI, Chowdhury F, Charles RC, LaRocque RC, Harris JB, Calderwood SB, Qadri F*, Ryan ET*. Circulating mucosal associated invariant T cells are activated in Vibrio cholerae O1 infection and associated with lipopolysaccharide antibody responses. PLoS Negl Trop Dis 2014; 8:e3076.