My laboratory has an NIH-funded program of International Collaborations in Infectious Disease Research, in collaboration with the International Centre for Diarrhoeal Disease Research in Dhaka, Bangladesh, to analyze human mucosal immune responses following natural V. cholerae infection, to analyze gene expression in V. cholerae directly in human samples, to use human immune responses following cholera to identify bacterial genes uniquely expressed during human infection, and to use this information to develop an improved cholera vaccine.
Selected recent references:
- Nelson EJ, Harris JB, Morris JG, Calderwood SB, Camilli A. Cholera transmission: the host, pathogen and bacteriophage dynamic. Nature Reviews Microbiology. 2009: 7:693-702.
- Patel SM*, Rahman MA*, Mohasin M, Riyadh MA, Leung DT, Alam MM, Chowdhury F, Khan AI, Weil AA, Aktar A, Nazim M, LaRocque RC, Ryan ET, Calderwood SB**, Qadri F**, Harris JB**. Memory B cell responses to Vibrio cholerae O1 lipopolysaccharide are associated with protection against infection in household contacts of cholera patients in Bangladesh. Clin Vaccine Immunol 2012: 19: 842-848.
- Harris JB, LaRocque R, Qadri F, Ryan ET, Calderwood SB. Cholera. Lancet 2012; 379: 2466-2476.
- Weil AA*, Chowdhury F*, Khan AI, Leung DT, Uddin T, Begum YA, Saha NC, Charles RC, LaRocque RC, Harris JB, Ryan ET, Qadri F**, Calderwood SB**. Frequency of re-exposure to Vibrio cholerae O1, evaluated by vibriocidal titer rise after one episode of severe cholera in a highly endemic area in Bangladesh. Am J Trop Med Hyg (in press).
- Johnson RA*, Uddin T*, Aktar A, Mohasin M, Alam MM, Chowdhury F, Harris JB, LaRocque RC, Bufano M, Yu Y, Wu Y, Leung DT, Sarracino D, Krastins B, Charles RC, Xu P, Kovac P**, Calderwood SB**, Qadri F**, Ryan ET**. Comparison of immune responses to the O-specific polysaccharide and lipopolysaccharide of Vibrio cholerae O1 in Bangladesh adult patients with cholera. Clin Vaccine Immunol (in press).