| Summary
This resource provides to MGH investigators a local
source of breeding pairs of the 22 chromosome substitution
strains (CSS)* of mice and access to basic phenotyping
equipment for mapping of simple or complex traits,
to promote the use of genetic mouse-based strategies
in basic and clinical research.
* Reference: Singer JB, Hill AE, Burrage LC, Olszens
KR, Song J, Justice M, O'Brien WE, Conti DV, Witte
JS, Lander ES, Nadeau JH. Genetic dissection of complex
traits with chromosome substitution strains of mice.
Science. 2004 304(5669):445-8.
Facilities
and Equipment
The CSS Resource comprises 22 mouse lines, each
homozygous for a single A/J chromosome (Chr 1-19,
X or Y and mitochondria) on a genetic background
that is otherwise C57BL/J that were generated by
Dr. Joseph Nadeau (Case Western Reserve University)
and his colleagues. The CSS mice are available from
The
Jackson Laboratories.
However, to save time and money, the MGH CSS Resource
maintains CSS breeding colonies that are housed in
the Simches-8 barrier facility, adjacent to a procedure
room that will have equipment for basic standardized
phenotyping, including behavioral and metabolic measurements.
Services
- Provide breeding pairs of each of the
22 CSS lines as a local resource, already acclimatized
to the MGH animal facility
- Provide access to basic mouse phenotyping
equipment and standardized protocols
- Promote the generation of genetic and phenotypic
datasets that can be cross-compared to generate mouse
trait maps
Director
Marcy E. MacDonald, Ph.D.
Center for Human Genetic Research
Massachusetts General Hospital
Richard B. Simches Research Center, CPZN-5414
185 Cambridge Street, Boston MA 02114
Phone Number: (617) 726-5089
macdonam@helix.mgh.harvard.edu
Staff
Edith Toral Lopez, B.A.
Phone: 617-726-5726
Fax: 617- 643-3202
etlopez@partners.org
Fee Schedule/Rates
Breeding pairs of CSS mice are provided at no cost
to interested investigators. Phenotyping capabilities
are being developed and input from interested investigators
is encouraged. Contact Marcy MacDonald at macdonam@helix.mgh.harvard.edu
Getting Started
To access the Resource, please contact Marcy MacDonald
at 617-726-5089 or by email at macdonam@helix.mgh.harvard.edu.
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