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Henry K. Beecher Pharmacology Laboratory
Summary
Science
Features
Research Strategies and Techniques
To investigate how general anesthetics affect ligand-gated ion channels, we study "artificial synapses" using submillisecond superfusion switching and patch-clamp electrophysiology. The effects of anesthetics on agonist-stimulated currents are analyzed using kinetic models. We also use Xenopus oocytes and two-microelectrode electrophysiology to study drug effects on ion channels with low time resolution. To get information about where anesthetics interact with ligand-gated ion channels to produce their effects, we create structural changes in cloned/expressed ion channels using molecular biological techniques. These mutated channels are investigated using the same electrophysiological techniques used to characterize wild-type receptors. |
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Office Information
Department of Anesthesia,
Critical Care and Pain Medicine
Gray-Bigelow 444
55 Fruit Street
Boston, MA 02114
Public Transportation Access: Yes
Disabled Access: Yes
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