Massachusetts General Hospital Department of Anesthesia, Critical Care and Pain Medicine - Critical Care Research
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Research Overview

Critical Care Research

Pain and Neurosciences Research

Biomedical Innovation

Ethics and Health Policy

Clinical Research

Grant Funding

 
 

Cardiac Critical Care Research Program

Summary Science Features

To investigate the role of cardiac and bone-marrow-derived TLR4 signaling in ischemic injury and to define the cellular and molecular mechanisms that govern such processes, we employ several mouse models of ischemic myocardial injury and a variety of biochemistry, molecular biology, and physiology techniques as described below.

TOLL-LIKE RECEPTOR 4 (TLR4) SIGNALING PATHWAYS

MOUSE MODELS OF ISCHEMIC CARDIAC INJURY
In vivo: Thoracotomy and coronary artery ligation
Ex vivo: Langendorff model in isolated hearts
In vitro: Hypoxic cardiomyocyte cultures
GENETIC MANIPULATION
 
Knock-out mice: TLR4-/-, TLR2-/-, MyD88-/-, IRAK-1-/-, iNOS-/-
Adenovirus (Ad)-mediated transgene expression: Ad.MyD88-wt, Ad.MyD88-dn, Ad.IRAK-1-wt,
Ad.IRAK-1-kd (kinase-deficient)
Chimeric models:

KO -> WT, WT -> KO
(Donor -> Recipient bone marrow transplantation)

CARDIAC FUNCTION ASSESSMENT

END POINTS



 
Office Information

Department of Anesthesia,
Critical Care and Pain Medicine
Gray-Bigelow 444
55 Fruit Street
Boston, MA 02114

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