Microscopy
 
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Zeiss LSM510 confocal
Zeiss Axio Observer 
Zeiss A1 Fluorescence
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Image Analysis
Zeiss LSM510 laser scanning confocal microscope

Location: CNY 149, Room 5.214E

Zeiss Axio Observer epi-fluorescence microscope (inverted)
Up to eight channel sequential acquisition, and up to four channel simultaneous acquisition.

Objectives
10x Zeiss Plan-NEOFLUAR air, 0.3NA
20X Zeiss Plan-APOCHROMAT air, 0.8NA
40X Zeiss C-APOCHROMAT UV-Vis-IR water, 1.2NA
40X Zeiss Plan-APOCHROMAT Corr D air, 0.95NA
63X Zeiss Plan-APOCHROMAT DIC oil, 1.4NA
100X Zeiss Plan-APOCHROMAT DIC oil, 1.4NA
100X Zeiss Plan-APOCHROMAT DIC oil, 1.46NA (high resolution/TIRF)
Laser Lines (Excitation Wavelengths)
405 nm: Diode laser, 50 mW
458, 477, 488 and 514 nm: Argon-ion laser, 30 mW
561 nm: Diode laser, 1 mW
633 nm: Helium-Neon laser, 15 mW
Primary Dichroic Filters
NT 80/20
HFT 405/488/561/633/KP 725
HFT 405/488/561
HFT 405/514/633
HFT 458/514/561
HFT 458/561
HFT 458
HFT 488
Secondary Dichroic Filters
NFT490, NFT515, NFT565, NFT600, KP560
NFT490, NFT515, NFT565
Barrier (Emission) Filters
Ch 1) LP420, LP505, LP575, LP650, BP575-615 IR, BP575-630 IR, BP600-650
Ch 2) LP420, LP475, LP505, BP420-480, BP475-545 IR, BP530-600, BP565-595, BP575-615 IR
Ch 3) LP505, LP530, LP575, BP505-530, BP500-545 IR, BP530-600, BP565-595, BP575-615 IR
Ch 4) BP530-600, BP575-615 IR, BP575-630 IR, BP600-650, LP530, LP575, LP600, LP650
Environmental control

Incubator XL.S1 LSM
Zeiss TempModule S1
Zeiss Stage Heating Insert P S1
Zeiss CO2 module S1
Heating device humidity S1
Accessories
Ludl motorized scanning stage 130x100 DC
X-CITE 120XL metal halide lamp
Software
Zeiss “Zen” 2008 LSM Imaging Software