Microscopy
 
Introduction
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Zeiss LSM510 confocal
Zeiss Axio Observer 
Zeiss A1 Fluorescence
Zeiss Mirax Midi Scanner
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Education
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Education
Microscopy Training Classes

A Microscopy Fundamentals training class is held on a regular basis or based on demand. Users are required to register for the training class by sending an email tdiefenbach@partners.org with a grant number, PI name, and contact information. The training fee is deducted from the first hour of microscope use time. Each class is 1.5 hours long and provides a general introduction to the principles and applications of microscopy, including fluorescence microscopy and confocal imaging.

Imaging System-Specific Training

Individual Training on the Microscopy microscopes is also provided. Users have a choice of the Zeiss LSM 510 confocal, the Zeiss/RPI high speed epifluorescence system, or a simple manual fluorescence microscope with color CCD camera and simple image capture software. Please contact tdiefenbach@partners.org to schedule individual training. A quick reference guide to the operation of the LSM510 confocal microscope may be found here.

Essential Reference Sites for Beginning to Advanced Microscopy

Both Nikon and Olympus offer comprehensive educational resources for every conceivable type of microscopy.
http://www.microscopyu.com/
http://www.olympusmicro.com/

Fluorescence Spectra References

Invitrogen has a fluorescence spectra viewer with a comprehensive list of fluorophores used in fluorescence microscopy:
http://www.invitrogen.com/site/us/en/home/support/Research-Tools/Fluorescence-SpectraViewer.html

BD Biosciences also has a fluorescence spectra viewer suited to spectra of flow cytometry fluorophores: http://www.bdbiosciences.com/colors/fluorescence_spectrum_viewer//