Flow Cytometry
 
Introduction
Training
Equipment
 
 
 
 
Core documents
Flow Links
Further Reading

Training
Just the FACS

The next session of the Ragon Institute's Introduction to Flow Cytometry class will be held Friday, January 27, 2012 at 12 pm in CNY149 room 5216, and typically lasts 90 minutes.
To get to 5216, take central elevators to the 5th floor, go through the door to the left of the balcony, and go down the hall to the right. 5216 is just before the billboard on the wall.

There will be a fee of $95 per person. When reserving your spot, please provide:
-- a grant/fund number.
--the TITLE of the fund, and if it is an NIH fund, the NIH number.
--The FULL name of the PI on the fund.

Email mwaring@partners.org if you would like to attend, and please view the "For Starters" tutorials linked below.
Download the class presentation (pdf).
This is a large file, please allow time for it to open. 90+ slides, so choose "multiple slides per page" if printing.
Flow Cytometry Syllabus
Below you will find a recommended sequence of resources to learn about Flow Cytometry.
For starters
  Tutorials on fluorescence, spectra, optical filters, flow, and data analysis from Invitrogen
  An online tutorial and quiz on compensation from Dr. Mario Roederer
Further introductory material
  Introductory tutorial from BD Biosciences (recently updated)
  A video discussion of compensation from Treestar, the makers of Flowjo software
Software Tutorials
  BD Biosciences (Index of files here or go to "Support>Training>e-Learning") :
  BD's online software tutorial for FACSDiva v6 on the LSRs (works in Explorer, I could not get it to work in Firefox. Complete Introduction, Overview, LSRII, and Data Analysis)
  CellQuest Pro Software Acquisition Tutorial
  CellQuest Pro Analysis Tutorial
  Flowjo:
  Flowjo PDF tutorial
  Flowjo online interactive tutorial (may take a while to load)
  Additional tutorial materials are available on the iMac analysis station
  Others:
  Modfit tutorial (cell cycle) (scroll to the bottom of the page)
Other Presentations

http://www.isac-net.org/
Select "Educational resources" from the menu on the left and then click on "Presentations" to find a vast resource of presentations from other groups. The "Tutorials from CYTO 2010" has many great presentations as well.

last updated 1/11/12