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| Flow Cytometry |
| Further Reading | ||
Useful papers and technical documents that everyone should read: |
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Nature Immunology Volume 7 Number 7 July 2006, p681-685 "Interpreting flow cytometry data: a guide for the perplexed." L.A. Herzenberg et al |
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BD Application note |
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“Flow Cytometry Controls, Instrument Setup, and the Determination of Positivity”, H.T. Maecker and J. Trotter, Cytometry Part A 69A:1037-1042 (2006) (You need an account for online access. There is a copy posted on the flow bulletin board in Room 5233). | |
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Minimum information for Flow Cytometry Data publication or go to the ISAC main page and select "Standards" and then "Current Standards" | |
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Using BD Compbeads | |
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Alternative to trypsin for adherent cells: The original thread mentioning the product on the Purdue list, they are now carried by CellSeed Inc Labware | |
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Quality Assurance for polychromatic flow cytometry (Steve Perfetto) | |
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A discussion of MFI | |
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PUCL index to advanced methodologies links to documents dealing with apoptosis, cell death, proliferation, immunophenotyping, and many more topics of advanced flow cytometry. | |
| Purdue List Summaries | ||
| Purdue University maintains a message board for flow cytometry, and there are several good threads that have been collected and can be found here | ||
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Summaries of popular flow topics | |
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Ways to minimize cell loss during washing | |
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Fixation to inactivate HIV (we ask you to use 2% fresh PFA for 2 hours). Please note the first article referenced, which shows that 1% PFA is INSUFFICIENT to fully inactivate HIV. | |
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Sorting of live human lymphocytes | |
| Flow Cytometry Books | ||
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Purdue University listing | |
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Practical Flow Cytometry, 4th edition | |
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Flow Cytometry Data Analysis: Basic Concepts and Statistics, James Watson |
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| Tissue Dissociation | ||
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Tissuedissociation.com | |
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Miltenyi Biotec gentleMACS | |
| Cell Cycle and Apoptosis | ||
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Invitrogen dyes for cell cycle and apoptosis | |
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NCIFCRF apoptosis | |
| Last updated 9/12/11 | ||