Flow Cytometry
 
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FACSCalibur

Our FACSCalibur is an analog system that runs Cellquest Pro and can detect up to 4 colors using 2 lasers (15 mW 488nm blue and 30 mW 633nm red). It has a fixed filter configuration, allowing you to detect fluorescent signal in the channels shown below. A User guide with tips and guidelines for instrument use can be found here. This instrument is located in CNY 149, Room 5104.

488 excitation

530/30 (FITC, GFP, Alexa 488)
575/26 (PE, PI)
670LP (PerCP)
635 excitation
  660/10 (APC)
Rates
  User-run analysis is billed at $40 per hour for all users.
Scheduling Instrument Time

To sign up for time on the Calibur, use the calendars hosted on www.schedulebook.com. A user account is required for access, to request one please email mwaring@partners.org

 

Last updated 9/12/11